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KMID : 0613820040140060913
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Journal of Life Science
2004 Volume.14 No. 6 p.913 ~ p.919
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Mitochondrial DNA Mutation (3243A→G, 1555A→G, 7445A→G) in Noise-Induced Hearing Loss
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Hong Young-Seoub
Hisahide Nishio
Lee Myeong-Jin
Kwak Ki-Young
Hwang Chan-Ho
Shin Dong-Hoon
Kwak Jong-Young
Lee Yong-Hwan
Kim Jong-Min
Kim Joon-Youn
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Abstract
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Mitochondrial DNA mutations have been reported in recent years in association with sensorineural hering loss. The purpose of this study is to identify the association between the noise-induced sensorineural hearing loss and the A to G mutation at nucleotide 3243, 1555, 7445 of mitochondrial DNA. Study subjects were established by history and chart review, and audiological and clinical data were obtained. Blood was sampled from 214 normal controls, 102 noise-induced hearing loss, and 28 sensorineural hearing loss. The DNA of these individuals were extracted, and mitochondrial DNA fragments were analyzed by polymerase chain reaction. Subsequently, the coding sequence of mitochondrial DNA 3243, 1555, 7445 were sequenced, and compared to the normal sequence, and all sequence variations were analyzed by restriction enzymes. Mitochondrial DNA mutations (3243A→G, 1555A→G, 7445A→G) were not detected by polymerase chain reactions in any patients with noise-induced hearing loss, sensorineural hearing loss, and normal controls. The DNA sequencing of PCR products did not revealed an A to G substitution at nucleotide 3243, 1555, 7445 of mitochondrial DNA. The noise-induced sensorineural hearing loss was not associated with mitochondrial DNA mutation (3243A→G, 1555A→G, 7445A→G).
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KEYWORD
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Mitochondrial DNA mutation, Noise-induced hearing loss
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